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Mechanism and theories of stomatal opening and closing is taught in the note for Gaseous Exchange in Plants. Read it below and look for the section on this topic.

Gaseous Exchange in Plants

10 min read

Stomata and gas exchange

Plants exchange gases through tiny pores called stomata, mostly on the underside of leaves. In daylight, carbon dioxide diffuses in for photosynthesis and oxygen diffuses out. At night, oxygen goes in for respiration. Water vapour leaves through the stomata during transpiration.

Inside a leafA cross-section of a leaf showing layers from the top: upper epidermis, palisade layer packed with chloroplasts, spongy layer with air spaces, and lower epidermis with a stoma between two guard cells where gases enter and leave.sunlightcarbon dioxide inoxygen outupper epidermispalisade layer: most chloroplastsspongy layer: air spacesstoma between two guard cellsvein (xylem and phloem)

How stomata open and close

Each stoma is surrounded by two guard cells. Their inner walls are thick and the outer walls thin, so when they take in water and become turgid they bend apart and the stoma opens. When they lose water they become flaccid and the stoma closes.

Guard cells and the stomaTwo pairs of guard cells. In light, potassium ions and then water enter the guard cells, which become turgid and curve apart, opening the stoma so that carbon dioxide can enter and water vapour leave. In the dark or when the plant is short of water, the guard cells lose water, become flaccid and close the stoma.Open (light, turgid)water in, K⁺ inClosed (dark, flaccid)water outCO₂ enters, water vapour leavesless water is lost

Theories of stomatal opening

TheoryIdea
Starch–sugarIn light the starch in guard cells is changed to sugar, which lowers their water potential; water enters and the cells swell. At night the sugar is changed back to starch.
Potassium ion (active)Light makes the guard cells pump K⁺ ions in. This lowers the water potential, so water enters by osmosis and they swell.
Abscisic acidWhen the plant is short of water, the hormone abscisic acid causes K⁺ ions to leave the guard cells; water follows and the stomata close.
Try itStomatal opening

The pore opens wider as light gets brighter. Water loss follows the pore width.

Check yourself What makes the stoma open?
Check yourself Which hormone closes stomata in dry conditions?
Check yourself What is pumped into guard cells in the light?

Key terms

Stoma
A pore in the leaf surface for gas exchange.
Guard cell
One of a pair of cells that controls the stoma.
Turgid
Full of water and firm.
Flaccid
Having lost water and soft.

Summary

  • Gases enter and leave leaves through stomata.
  • Guard cells open the pore when turgid and close it when flaccid.
  • Theories: starch–sugar, potassium ion pumping, and abscisic acid.
  • Stomata open in the light and close in the dark or in drought.